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Patent No. US 08137906
Issue Date Mar 20, 2012
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Patent 08137906 - Method for the synthesis of DNA fragments > Summary

US Patent

Patent No. US 08137906

Published on Mar 20, 2012

Application No. 11/336198

Filed on Jan 20, 2006

Priority Date -

Inventor/Applicants

Schatz, Octavian - Altomunster, DE (7)

Abstract

The invention relates to a method that can be carried out in parallel and automated for the production of any nucleic acid, comprising the following steps: a) coupling an oligonucleotide to a solid matrix b) adding an additional oligonucleotide c) ligating the oligonucleotides from steps a) and b) in one orientation d) removing excess reactants and enzymes from the reaction preparation e) cleaving the ligation product from step c) with a restriction enzyme that cleaves outside the recognition sequence, whereby cleavage occurs in the oligonucleotide from step a) or in the oligonucleotide from step b) f) separating the reaction mixture from the lengthened or shortened oligonucleotide from step a) that is obtained in step e) g) repeating steps b) to f) at least once h) successive sequence-independent linkage of the fragments obtained after performing steps a) to g) until the desired product is obtained.

Classification

International: 5

C12Q 1/68

C12P 19/34;

C07H 21/02;

C07H 21/04;

C07H 21/00;

National: 6

435/6

435/91.1;

536/23.1;

536/24.3;

536/24.33;

536/25.3;

Field of Search 9

435/6

435/91.1;

435/183;

436/94;

536/23.1;

536/24.3;

536/24.33;

536/25.3;

536/25.32;

Examiner

Lu, Frank W